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Ultra-deep sequencing reveals intra-host diversity and selective constraint in SARS-CoV-2 samples from Chile

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By Carol Moraga, Barbara Bernal, Sofia Poblete, Adrian Gonzalez, Adrian González, Mauricio Latorre, Alex Di Genova, Francisco Kirhman
| Nature Verified
Abstract SARS-CoV-2 genomic surveillance has mainly focused on consensus genomes, but low-frequency variants within infected individuals can provide additional insight into viral diversity, mixed infections, and early evolutionary processes. Here, we performed ultra-deep whole-genome sequencing of 96 SARS-CoV-2 samples collected in Chile between 2020 and 2022 using the MGI ATOPlex platform and DNBSeq-G400 sequencing.
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