Highlights • Multiple BcsD cryo-EM structures reveal distinct quaternary structure assemblies • BcsD- and proline-rich partners assemble BcsD fold-specific cytoskeletal scaffolds • BcsD-partner scaffolds interact directly with the cellulose synthase complexes • Resultant synthase arrays may determine polymer properties and biofilm architecture Summary Cellulose is the world’s most abundant biopolymer, and similar to its role as a cell wall component in plants, it is a prevalent constituent of...