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Iterative and data-driven ortholog mining enables reliable discovery of stereoselective ketoreductases

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By Peter Stockinger, Michael Niklaus, Kevin Yar, Steven Hanlon, Hans Iding, Rebecca Buller, Nicolas Imstepf, Ivana Pastierikova, Jasmin Küng, Sumire Honda Malca
| Nature Verified
Abstract Ketoreductases (KREDs) enable stereoselective alcohol synthesis and are widely used industrially. Yet identifying process-compatible and selective enzymes for non-native substrates remains challenging, requiring extensive screening. Here, we present an iterative ortholog mining strategy to explore functional space.
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