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Identifying and antagonizing the interactions between layilin and glycosylated collagens
Highlights • Affinity purification/mass spectrometry shows that layilin binds glycan-rich collagens • Calcium ion binding can be leveraged to engineer epitope-specific antibodies • Calcium-dependent and -independent antibodies have different binding properties Summary Layilin is a small type I transmembrane receptor thought to bridge extracellular ligands with the cytoskeleton through its intracellular interactions with the scaffolding protein talin.
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