Abstract To meet the escalating food and fuel demands of a growing global population and industry, food production requires a 50% increase by 2050. However, various environmental stresses, such as excessive light, significantly inhibit plant growth and lead to substantial reductions in crop yields. A major contributing factor to such declines is the reduction in photosynthetic capacity. In this study, a chemical-screening system based on standard 96-well plate and tobacco leaf tissue was developed.