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Tadayoshi Kanao on Muck Rack

Tadayoshi Kanao

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Articles

Dual-calibration RT-qPCR reveals distinct dnaK1/2/3 and groEL1/2 expression dynamics under heat and acid stress in the sinefungin producer Streptomyces incarnatus

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By Tomohisa Hasunuma, Takashi Tamura, Zhao Xiao-Hui, Mao Kubo, Haruka Yamagata, Yuriko Nakashima, Tadayoshi Kanao
| Nature Verified
Abstract Streptomyces incarnatus NRRL8089 produces sinefungin, a nucleoside antibiotic whose yield is enhanced under defined environmental stresses. Here, dual-calibration RT-qPCR was used to resolve paralogue-specific expression dynamics of the dnaK and groE chaperone systems under heat and acid stress conditions that increase sinefungin titres.
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