Tania A. Stallons

MATERIALS AND METHODS AR42J rat pancreatic cell line was purchased from American Type Culture Collection. The cells were tested for Mycoplasma by Hoechst DNA stain, agar culture, and polymerase chain reaction–based assay by American Type Culture Collection and were not detected as per certificate of analysis. The cells were maintained in F12K medium (Gibco) containing 20% fetal bovine serum (Gibco). Athymic nude and CD-1 mice were purchased from Envigo.

MATERIALS AND METHODS The PC-3 prostate cancer cell line was purchased from ATCC. Cells were maintained in F12K medium (Gibco) containing 10% fetal bovine serum (Gibco). Athymic nude and CD-1 mice were purchased from Envigo. All experiments were conducted using male mice unless otherwise stated. Animal studies were approved by the Institutional Animal Care and Use Committee and performed in compliance with guidelines from the Public Health Service Policy and Animal Welfare Act of the United States.