Teeshyo Bhattacharya

Abstract Beginning with the pioneering hybridoma technology developed in 1975, antibody generation methodologies have advanced substantially, culminating in today’s single-cell techniques. Each successive approach contributes unique applications, advantages, and drawbacks that reflect the field’s dynamic progress. We highlight the impact of integrating single-cell RNA sequencing (scRNA-seq) with display technologies.

Decellularized extracellular matrix-based bioengineered 3D Breast cancer scaffolds for personalized therapy and drug screening Breast cancer is the second deadliest cancer after lung cancer. Like all cancers, breast cancer is also driven by a 3-D microenvironment. ECM is an essential component of the 3-D tumor micro-environment, wherein it functions as a scaffold for cells and provides metabolic support. Breast cancer is characterized by alteration in the ECM.