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An Optimized Method for the Proteomic Analysis of Low Volumes of Cell Culture Media and the Secretome: The Application and the Demonstration of Altered Protein Expression in iPSC-Derived Neuronal Cell Lines from Parkinson’s Disease Patients

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By Anna Baud, Daniel Little, Wendy Heywood, Teo Qi Wen
| American Chemical Society
Abstract Traditionally, cell culture medium in iPSC-derived cell work is not the main focus of the research and often is considered as just “food for cells”. We demonstrate that by manipulation of the media and optimized methodology, it is possible to use this solution to study the proteins that the cell secretes (the “secretome”). This is particularly useful in the study of iPSC-derived neurons, which require long culture time.
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