Thaddeus M. Barney

CONFLICT OF INTEREST STATEMENT The authors declare no conflicts of interest. Supporting Information Filename Description dev22442-sup-0001-SupMat.tif7 MB Supplemental Figure 1. An experimental schematic (A) details the training for Experiments 3 and 4 for male and female rats, with a similar training prior to test (also identical to the training in Experiment 2).

Introduction Alcohol exposure leads to rapid alterations in neuroimmune gene expression in the CNS (Doremus-Fitzwater and Deak, 2022). In acute exposure conditions, ethanol acts as a potent neuroimmune stimulus which mobilizes HMGB1 and potentially other ligands of toll like receptor (TLR) 4 and downstream activation of several immune signaling pathways such as NFκB and MAPK (Fernandez-Lizarbe et al., 2009; Pascual et al., 2015; Montesinos et al., 2018).