Thai Binh Pham

Abstract CRISPR-based gene drive systems have emerged as promising strategies for mosquito population control to reduce the impact of vector-borne diseases such as malaria. However, their broader molecular impact on the mosquito transcriptome remains poorly understood. We performed an RNA sequencing analysis of Anopheles gambiae gene drive (GD) mosquitoes under three conditions: males fed with sugar, females fed with sugar, and females fed with blood.

CRISPR/Cas9 gene-editing technology has transformed the field of genome modification. This system is composed of two fundamental components that interact to form a complex: Cas9 endonuclease and sgRNA, a target-specific RNA that guides Cas9 to the desired genomic DNA target site. Cas9 induces a double strand break at the target site, activating the DNA repair pathways of homology-directed repair (HDR) and nonhomologous end joining (NHEJ).