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Articles by Tilman E. Schäffer
Nanosecond pulsed electric fields induce cell-size-dependent selective permeabilization of urothelial cancer cells - Communications Biology
Abstract Plasma membrane integrity is vital for cell viability, yet its controlled disruption enables targeted delivery of therapeutic agents. Here, we examined membrane durability and repair capacity in normal and malignant urothelial cells using short, high-voltage nanosecond pulses. Pulses were applied to monolayer cultures, spheroids, and patient-derived organoids.
Quantifying and Utilizing Electroosmotic Flow for Mechanical Measurements with the Scanning Ion Conductance Microscope
Basics of EOF in the SICM Nanopipette In the SICM nanopipette, applying a positive voltage V0 to the nanopipette electrode relative to the bath electrode causes an outward EOF through the nanopipette ( Figure 1 a, red) due to the (usually) negatively charged glass surface of the (uncoated) nanopipette.
Differences in apical and basal mechanics regulate compliance of curved epithelia
Keywords polarity epithelial cells viscoelasticity tissue tension cellular cortex Introduction Epithelial monolayers form physical and chemical barriers and control the permeability between the internal and external environment. The function of these tissue sheets is often coupled to its considerable mechanical resilience.
Excessive endometrial PlGF- Rac1 signalling underlies endometrial cell stiffness linked to pre-eclampsia - Communications Biology
Abstract Cell stiffness is regulated by dynamic interaction between ras-related C3 botulinum toxin substrate 1 (Rac1) and p21 protein-activated kinase 1 (PAK1) proteins, besides other biochemical and molecular regulators. In this study, we investigated how the Placental Growth Factor (PlGF) changes endometrial mechanics by modifying the actin cytoskeleton at the maternal interface.
Measuring the Shape, Stiffness, and Interface Tension of Droplets with the Scanning Ion Conductance Microscope
Imaging and probing liquid–liquid interfaces at the micro- and nanoscale are of high relevance, for example, in materials science, surface chemistry, and microfluidics. However, existing imaging techniques are limited in resolution, average over large sample areas, or interact with the sample.
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