Abstract The orderly architecture and energy supply of mitochondria, orchestrated by proteins, are essential for oocyte maturation. Acylglycerol kinase (AGK) is known to predominantly localize to the mitochondrial membrane and regulate multiple cellular processes related to Sengers syndrome and tumorigenesis. However, the functions of AGK in the ovaries remain largely unknown. Here, we generate mice with oocyte-specific disruption of Agk.