Tingying Peng

Abstract Cryo-electron tomography provides unique insights into macromolecular complexes in their native environments, yet membrane analysis remains a major bottleneck due to low signal-to-noise ratios, missing wedge artifacts and the complexity of membrane-associated particles. Existing tools often require extensive manual annotation, struggle with generalization across datasets and lack integrated solutions for segmentation, particle localization and quantitative analysis.

Abstract Cryo-electron tomography allows us to visualize and analyze the native cellular environment on a molecular level in 3D. To reliably study structures and interactions of proteins, they need to be accurately localized. Recent detection methods train a segmentation network and use post-processing to determine protein locations, often leading to inaccurate and inconsistent locations.

Abstract Cell death, such as apoptosis and ferroptosis, play essential roles in the process of development, homeostasis, and pathogenesis of acute and chronic diseases. The increasing number of studies investigating cell death types in various diseases, particularly cancer and degenerative diseases, has raised hopes for their modulation in disease therapies.