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The study was conducted on fourteen edible bullfrogs between 3 December 2019 and 11 February at the National Zoological Garden (NZG), South African National Biodiversity Institute, Pretoria, South Africa (25.7469°S, 28.18918°E). All study animals were housed at the NZG as part of the animal collection since being confiscated from poachers collecting individuals within the wild in 2016 and are thus defined as adult.
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