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Tommy J Sroka on Muck Rack

Tommy J Sroka

As seen in: Nature, BioRxiv
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Articles

An enzymatic cascade enables sensitive and specific proximity labeling proteomics in challenging biological systems - Nature Communications

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By Tommy J Sroka, Lea K Sanwald, Avishek Prasai, Damian Weber, Peter Walentek, Kerstin Feistel, Valentina Trivigno, Valerie Chaumet, Louisa M. Krauß, Bianca Schrul
| Nature Verified
Abstract Ascorbate peroxidase (APEX) is a proximity labeling enzyme used for subcellular proteomics at high spatial and temporal resolution. However, toxicity of its substrate hydrogen peroxide and background labeling by endogenous peroxidases limit its use to in vitro studies of specific cell types.
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Proximity proteomics of primary cilia in human hypothalamic neurons

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By Viviana Macarelli, Tommy J Sroka, Jan N. Hansen, Ulrika Axelsson
| BioRxiv
Abstract Primary cilia are hair-like sensory organelles that project from the cell bodies of most cell types, including appetite-regulatory hypothalamic neurons where they likely help sense metabolic factors to regulate food intake. We hypothesized that characterising the proteins present in the primary cilia of hypothalamic neurons would shed mechanistic insights into their sensory role and identify new therapeutic targets for obesity.
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iAPEX: Improved APEX-based proximity labeling for subcellular proteomics using an enzymatic reaction cascade

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By Avishek Prasai, Tommy J Sroka, Lea K Sanwald, Josefine Hoeren
| BioRxiv
Abstract Ascorbate peroxidase (APEX) is a versatile labeling enzyme used for live-cell proteomics at high spatial and temporal resolution. However, toxicity of its substrate hydrogen peroxide and background labeling by endogenous peroxidases limit its use to in vitro studies of specific cell types.
Open in Who Shared Issue with article?
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