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Torrey W. Rodgers on Muck Rack

Torrey W. Rodgers

As seen in: BioRxiv
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Articles

Understanding detection probability in a low-density population to inform optimal eDNA sampling for freshwater mussels

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By Torrey W. Rodgers, Karen Mock
| BioRxiv
Abstract Environmental DNA (eDNA) offers a sensitive tool for detecting aquatic species, including those at low population densities. However, effective use of eDNA in conservation and monitoring efforts requires an understanding of detection probabilities and optimal replication effort necessary to minimize false negatives. This study assessed the probability of detecting Anodonta nuttalliana, a native freshwater mussel, in two Utah populations with contrasting densities (medium and low).
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A novel degenerate primer set for eDNA metabarcoding of amphibians, turtles and fish

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By Torrey W. Rodgers, John Olson, Charles Hawkins, Karen Mock
| BioRxiv
Abstract Metabarcoding of environmental DNA (eDNA) has revolutionized the detection of aquatic species across large geographic scales. However, the effectiveness of eDNA metabarcoding across taxa is marker-dependent, often requiring multiple markers to detect divergent taxonomic groups. Here, we introduce a novel metabarcoding marker designed to simultaneously detect amphibians, turtles, and fish from eDNA samples.
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Multiplex qPCR assays for detection of two imperiled anuran species, Anaxyrus californicus and Spea hammondii, from environmental DNA

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By Torrey W. Rodgers, James Walton, Daniel Prince Verified, Robert E. Lovich
| BioRxiv
New Results doi: https://doi.org/10.1101/2022.12.20.521282 Abstract We developed a species-specific, quantitative PCR assay multiplex for the detection of two threatened and endangered anuran species, arroyo toad (Anaxyrus californicus) and western spadefoot (Spea hammondii), from environmental DNA (eDNA). Both species have experienced dramatic declines over the last century throughout their ranges, mostly as a result of habitat loss.
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