Tusar Giri

Abstract Altered patterns of genetic expression induced by isoflurane preconditioning in mouse brain have not yet been investigated. The aim of our pilot study is to examine the temporal sequence of changes in the transcriptome of mouse brain cortex produced by isoflurane preconditioning. Twelve-wk-old wild-type (C57BL/6J) male mice were randomly assigned for the experiments.

Immune cell census and response to immune stimulation are altered in the pregnant lung Flow cytometric assessment of immune cell frequencies in single cell suspensions of the lung revealed an increase in pDCs in the pregnant lung (Fig 3A); frequencies of CD4+, CD8+, and NK cells were unchanged (S5 Fig of S1 File). Immune stimulation with resiquimod was associated with enhanced release of IFN-α and IFN-β in the lungs of both pregnant and non-pregnant rats (Fig 3B).

ORIGINAL RESEARCH Arvind Palanisamy, Corresponding Author arvind.palanisamy@wustl.edu orcid.org/0000-0001-8460-1759 Department of Anesthesiology, Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, Missouri, USA Correspondence Arvind Palanisamy, Department of Anesthesiology, Washington University School of Medicine, 660 S Euclid Ave, St. Louis, MO 63110, USA. Email: arvind.palanisamy@wustl.edu Nicole N.