Tyler A Sisley

Abstract Staphylococcus aureus is a Gram-positive pathogen responsible for antibiotic-resistant infections. To identify vulnerabilities in cell envelope biogenesis that may overcome resistance, we enriched for S. aureus transposon mutants with defects in cell surface integrity or cell division by sorting for cells that stain with propidium iodide or have increased light-scattering properties, respectively.

Correction to: Nature Biotechnology https://doi.org/10.1038/s41587-021-00938-z, published online 28 June 2021. In the version of the article initially published, in the “High-throughput DNA sequencing of genomic DNA samples” section of the Methods, the formula used to calculate product purity was incorrect, and has been updated to read “C•G-to-G•C editing yield ÷ (C•G-to-G•C editing yield + C•G-to-T•A yield + C•G-to-A•T yield + indels)” in the HTML and PDF versions of the article.

Abstract The peptidoglycan (PG) cell wall protects bacteria against osmotic lysis and determines cell shape, making this structure a key antibiotic target. Peptidoglycan is a polymer of glycan chains connected by peptide crosslinks, and its synthesis requires precise spatiotemporal coordination between glycan polymerization and crosslinking. However, the molecular mechanism by which these reactions are initiated and coupled is unclear.