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Articles

Extracellular vesicles from human plasma for biomarkers discovery: Impact of anticoagulants and isolation techniques

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By Valentina Bettio, Eleonora Mazzucco, Annamaria Antona, UPO Biobank
| PLOS ONE
Multiplex phenotyping of UC- EVs and AS-EVs To better characterize the surface marker profile of UC- and AS-EVs, we performed a multiplex bead-based cytometry (MACSPlex exosome plasma kit, Miltenyi Biotec), that allows the simultaneous detection and semi-quantitative analysis of 37 different EVs surface epitopes. The mean fluorescence intensity (MFI) of every single marker was analyzed as reported in material and methods.
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