Vanghelita Andrei

1 Introduction PLAG1 (pleomorphic adenoma gene 1) is located on chromosome band 8q12 and was first described in pleomorphic adenomas of the salivary glands [1]. It belongs to the PLAG family of transcription factors along with PLAG-like 1 and PLAG-like 2 genes. Rearrangement of PLAG1 is described in lipoblastoma (LB), myoepitheliomas/mixed tumors, uterine myxoid mesenchymal tumors, and the more recently described pediatric fibromyxoid soft tissue tumor [2-6].

Differential methylation of CpG sites and GO enrichment analysis Estimation of differentially methylated positions (DMPs) analysis was done by using the functions from the R-package ‘limma’ [22]. The tests were carried out on the M values of the top 10,000 CpG positions ranked by variability. Relevant CpGs were defined when adjusted p value was smaller than 0.01 and a logfold-change larger than 2. Multiple testing correction was performed by using Benjamini Hochberg.