VIB Proteomics Core

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An experimental in vivo dysbiosis challenge model was used as previously described by De Meyer et al. [5]. Briefly, a total of 96 day-old broiler chicks (Ross 308) were randomly assigned to challenge (Ctrl+) and control (Ctrl−) groups, and allocated in a pen-based housing with six birds in each pen (8 pens per group). The sample size was determined using the G*Power software (ver. 3.1.9.2, Kiel, Germany) with applied parameters of α = 0.05 and power = 95%.

All the experiments and manipulations with animals were approved by the ethical committee of the Faculties of Veterinary Medicine and Bioscience Engineering of Ghent University (EC2020-045). Housing and animal care was in accordance with the Royal Decree of 13 June 2010 following EU Directive 2007/43/EC of 28 June 2007. The experimental setup of the study is detailed in Figure 1. Graphical abstract of the study. This figure was created with Biorender.com and exported under a paid subscription.