Victor D Pivovarov on Muck Rack

Victor D Pivovarov

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Articles

Comparative diagnostics of allergy using quantitative immuno-PCR and ELISA

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By Dmitry Ryazantsev, Maria A Simonova, Victor D Pivovarov, Anna S Dolgova
| Bioanalysis Verified
Aim: Estimation of specific IgE is essential for the prevention of allergy progression. Quantitative immuno-PCR (qiPCR) can increase the sensitivity of IgE detection. We aimed to develop qiPCR and compare it to the conventional ELISA in identification of IgE to Alt a 1 and Fel d 1 allergens. Results: Single stranded 60-mer DNA conjugated to streptavidin was used to detect antigen–IgE–biotin complex by qiPCR.
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