Warin Rangubpit

Citation: Sangthong D, Sangthong P, Rangubpit W, Pongprayoon P, Suwan E, Wongpanit K, et al. (2025) Molecular characterization of Rhipicephalus microplus and Haemaphysalis bispinosa ticks from cattle across Thailand: Regional identification and evidence of different genetic sub-structures between mainland and peninsular populations. PLoS One 20(11): e0337052. https://doi.org/10.1371/journal.pone.0337052 Editor: Shawky M.

Abstract RyR1 is an intracellular Ca2+ channel important in excitable cells such as neurons and muscle fibers. Ca2+ activates it at low concentrations and inhibits it at high concentrations. Mg2+ is the main physiological RyR1 inhibitor, an effect that is overridden upon activation. Despite the significance of Mg2+-mediated inhibition, the molecular-level mechanisms remain unclear.

Abstract RyR1 is an intracellular Ca2+ channel important in excitable cells. Ca2+ activates it at low concentrations and inhibits it at high concentrations. Mg2+ is the main physiological RyR1 inhibitor, an effect that is overridden upon activation. Despite the significance of Mg2+-mediated inhibition, the molecular-level mechanisms remain unclear. We determined two cryo-EM structures of RyR1 with Mg2+ up to 2.8 Å resolution, identifying multiple Mg2+ binding sites.