Wei Yuan Cher

Abstract Individual genetic polymorphisms can exert a modifier effect on splicing. With about one single-nucleotide polymorphism per 250 bases, we hypothesized that consideration of splice haplotypes will improve the accuracy of spliceogenic variant annotation, as current methods mainly assess single DNA variants and in a single reference genome.

Abstract MINORg is an offline gRNA design tool that generates the smallest possible combination of gRNA capable of covering all desired targets in multiple non-reference genomes. As interest in pangenomic research grows, so does the workload required for large screens in multiple individuals. MINORg aims to lessen this workload by capitalising on sequence homology to favour multi-target gRNA while simultaneously screening multiple genetic backgrounds in order to generate reusable gRNA panels.