Wenguang Yin

Wenguang Yin, Hyun-Taek Kim, ShengPeng Wang, Felix Gunawan, Rui Li, Carmen Buettner, Beate Grohmann, Gerhard Sengle, Debora Sinner, Stefan Offermanns, Didier Y.R. Stainier European Respiratory Journal 2019 53: 1800840; DOI: 10.1183/13993003.00840-2018 Abstract Epithelial tubes, comprised of polarised epithelial cells around a lumen, are crucial for organ function. However, the molecular mechanisms underlying tube formation remain largely unknown.

Wenguang Yin, Hyun-Taek Kim, ShengPeng Wang, Felix Gunawan, Rui Li, Carmen Buettner, Beate Grohmann, Gerhard Sengle, Debora Sinner, Stefan Offermanns, Didier Y.R. Stainier European Respiratory Journal 2018; DOI: 10.1183/13993003.00840-2018 Abstract Epithelial tubes, comprised of polarised epithelial cells around a lumen, are crucial for organ function. However, the molecular mechanisms underlying tube formation remain largely unknown.

We sought to identify regulators of mouse respiratory organ formation by conducting a large-scale forward genetic screen using ethylnitrosourea (ENU) mutagenesis. Mutagenized C57BL/6J mice were bred to uncover recessive phenotypes using a two generation backcross breeding scheme (Supplementary Fig. 1a). Early postnatal tracheas were examined using alcian blue to label cartilage, and alpha SM actin (αSMA) immunostaining in whole-mount preparations (Fig. 1a).