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Wiktor Antczak

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Articles

Degron models: a toolbox for rapid in vivo depletion of essential proteins regulating mRNA metabolism

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By Wiktor Antczak, Marcin Szpila, Vanessa Linke, Andrzej Dziembowski, Olga Gewartowska, Katarzyna Sałas, Patrycja Daszczuk
| Nature Verified
Abstract Due to their essentiality, studying proteins involved in fundamental processes in vivo is challenging. PROTAC-based systems offer time-controlled protein depletion, but their characterization in vivo remains limited. Here, with an efficient direct zygote editing protocol, we generate degron-tag models (dTAG/FKBP or BromoTag) for seven genes involved in therapeutic and endogenous mRNA metabolism (Cnot1, Pan2, Tent5a, Tent4b, Dcp2, Rnasel, Tsg101).
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Re-adenylation by TENT5A enhances efficacy of SARS-CoV-2 mRNA vaccines - Nature

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By Paweł S. Krawczyk, Wiktoria Orzeł, Wiktor Antczak, Karolina Kasztelan, Aleksandra Brouze, Natalia Gumińska, Agnieszka Tudek, Tomasz Spiewla, Dominika Nowis, Joanna Kowalska, Jacek Jemielity, Andrzej Dziembowski
| Nature Verified
Abstract Despite the widespread use of mRNA vaccines against COVID-19, little is known about the metabolism of therapeutic RNAs. Here we use nanopore sequencing1,2,3 to analyse individual therapeutic mRNA molecules, focusing on their poly(A) tails. We show that the Moderna mRNA-1273 vaccine4 has a poly(A) tail of around 100 nucleotides, followed by an mΨCmΨAG sequence. In cell lines, mRNA-1273 undergoes rapid degradation initiated by mΨCmΨAG removal, followed by CCR4–NOT-mediated deadenylation.
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