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Control of ice thickness in cryo-EM via confinement
Abstract Achieving thin ice with optimal thickness is a prerequisite for successful cryo-EM analysis of embedded macromolecules. Despite decades of effort, precise control of ice thickness in cryo-EM remains a major challenge. Here we reveal that the intrinsic instability of sub-100-nm liquid film, a previously overlooked determinant before vitrification, plays a critical role in controlling ice thickness.
Nuclease‐Mimetic Nanomaterials: From Fundamentals to Bioapplications
Conflict of Interest The authors declare no conflict of interest. References 1, , , , , Nat. Rev. Cancer 2022, 23, 78. 2, , Polym. J. 2012, 44, 929. 3, , , DNA Repair 2018, 66–67, 24. 4, , , , , , , , Cancer Res. 2023, 83, 1234. 5, , , , , , Nucleic Acids Res. 2020, 48, 3975. 6, , , , Chem. Lett. 2019, 48, 1332. 7, , , , , , J. Am. Chem. Soc. 2021, 143, 9724. 8, , , , , , Angew. Chem., Int. Ed. 2022, 61, 202113103. 9, , , , , , , J. Phys. Chem. Lett. 2022, 13, 10929. 10, , , , , , , , , Nat. Rev. Mater. 2022, 7, 557.
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