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Xinxun Yang

As seen in: Wiley Online Library, BioRxiv
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Articles

Boosting High‐Fidelity Isotropic Super‐Resolution via Image Interference Structured Illumination Microscopy with Spatial‐Spectral Optimization

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By Enxing He, Yile Sun, Hongfei Zhu, Xinxun Yang
| Wiley Online Library Verified
Data Availability Statement The data that support the findings of this study are available from the corresponding author upon reasonable request.
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I2SIM: Boosting High-Fidelity Isotropic Super-Resolution with Image Interference and Spatial-Spectrum Optimization

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By Yile Sun, Hongfei Zhu, Xinxun Yang, Enxing He
| BioRxiv
Abstract Spatial resolution is crucial for imaging subcellular structures. The advent of three-dimensional structured illumination microscopy (3D-SIM) greatly benefits the biology community, providing a powerful tool for imaging organelles with a two-fold resolution enhancement in all three dimensions. However, the axial resolution of 3D-SIM is limited to around 300 nm, which is inferior to its lateral resolution.
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Accurate 3D SMLM localization via Vectorial In-situ PSF Retrieval and Aberration Assessment

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By Xinxun Yang, Hongfei Zhu, Yile Sun, Hanmeng Wu
| BioRxiv
Abstract In single-molecule localization microscopy (SMLM) experiments, achieving precise localization hinges on obtaining authentic point spread function (PSF) influenced by system and sample-induced aberrations. Here, we introduce VISPR (Vectorial in-situ PSF retrieval) as a method of retrieving precise 3D PSF model considering both system and sample-induced aberrations under SMLM conditions.
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