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Xuliu Zhou on Muck Rack

Xuliu Zhou

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Articles

Fibrillarin-mediated 2’-O-methylation serves as a translation brake on uncapped enterovirus RNA

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By Kui Zhang, Baocheng Yu, Xuliu Zhou, PLOS Pathogens
| PLOS
Supporting information (A) Overlap of Nm sites (methylation rate ≥ 0.1) identified across triplicate virus infected samples by NanoNm. (B) The assay was firstly validated using a 100-nt synthetic HIV RNA probe (P-2’O, 733–832 nt) containing known Am783 (position 51) and artificial Cm (position 21) sites. (C) Schematic workflow, including low-dNTP reverse transcription, template-independent poly(C) tailing, PCR amplification with adapter primers, and TA cloning for sequencing validation.
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