Yan-Ying Li

1 Introduction The acidification of heavy metal mine tailings represents a significant environmental challenge (Park et al. 2019). Pyrite, as the most globally abundant metal sulfide mineral, is widely recognised as a major contributor to acid mine drainage (AMD) (Chandra and Gerson 2010; Fan et al. 2017). Once exposed to air, pyrite can be oxidised through the combined action of oxygen and water, producing sulfuric acid and releasing heavy metal ions from tailings.

Conflicts of Interest The authors declare no conflicts of interest. Supporting Information Filename Description bmc70105-sup-0001-Supplementary_Material.docxWord 2007 document , 106.1 MB Figure S1 BPI of V. coloratum in the negative ion mode. Figure S2 Representative foot photos of each group of rats. Figure S3 BPI of normal rats in blank and V. coloratum metabolic samples in the negative ion mode. Figure S4 Base peak intensity chromatograms (BPIs) of RA rats in blank and V.