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Yin-Hsi Lin on Muck Rack

Yin-Hsi Lin

As seen in: Nature, BioRxiv
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Articles

A high-affinity split-HaloTag for live-cell protein labeling

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By Yin-Hsi Lin, Julian Kompa, De-en Sun, Runyu Mao, Birgit Koch, Konstantin Hinnah, Jonas Wilhelm, Natascha Franz, Stefanie Kühn, Tanja Menche, Abdinasir Adow, Julien Hiblot, Kai Johnsson, Paula Breuer
| Nature Verified
Abstract We introduce a high-affinity split-HaloTag comprised of a short peptide tag (Hpep, 14 residues) and a large, inactive fragment (cpHaloΔ3). Hpep binds to cpHaloΔ3 spontaneously with nanomolar affinity, enabling subsequent labeling with fluorescent HaloTag ligands.
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Improving Split-HaloTag through Computational Protein Engineering

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By Jonas Wilhelm, Lennart Nickel, Julien Hiblot, Yin-Hsi Lin
| BioRxiv
Abstract Split-HaloTag can be used to transform transient molecular interactions into permanent marks through chemical labeling, thereby enabling the recording of transient physiological events in individual cells. However, applications of split-HaloTag-based recorders can be limited by slow labeling rates. To address this issue, we have engineered an improved version of cpHaloΔ, the larger fragment of the split-HaloTag system.
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