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Ying-Mi Lai on Muck Rack

Ying-Mi Lai

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TIMAHAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics

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By Chin-Wen Chen, Pei-Yi Lin, Miao-Hsia Lin, Ying-Mi Lai
| BioRxiv
Abstract Protein post-translational modifications (PTMs) are crucial in plant cellular processes, particularly in protein folding and signal transduction. N-glycosylation and phosphorylation are notably significant PTMs, playing essential roles in regulating plant responses to environmental stimuli. However, current sequential enrichment methods for simultaneous analysis of phosphoproteome and N-glycoproteome are labor-intensive and time-consuming, limiting their throughput.
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