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Ethanol inhibits the growth and metastasis of hepatocellular carcinoma by inducing immunogenic cell death
Materials and methods C57BL/6 and BALB/c mice were obtained from the National Laboratory Animal Center (NARLabs, Taipei, Taiwan). The two mouse HCC cell lines (Hep-55.1C and BNL 1ME A.7R.1) and the two human HCC cell lines (HepG2 and PLC/PRF/5) were used in this study. To determine the viability of each HCC cell line after treatment with low-concentration ethanol, cells were cultured in the absence or presence of graded concentrations of ethanol for 24 and 48 hours.
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