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Hydrogen–Deuterium Back-Exchange for High-Resolution Mass-Spectrometry-Based Metabolite Characterization Click to copy article link Article link copied!
Abstract Click to copy section linkSection link copied! Methods for unknown metabolite characterization rely heavily on one or more of the following: spectroscopic approaches; tandem mass spectrometry (MS/MS); ion mobility or liquid chromatography; and/or guided intervention through reverse metabolomics, derivatization, etc. The limitations of these methodologies vary widely. For example, spectroscopic approaches are slow and limited in throughput as they necessitate metabolite purification.
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