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Exploiting functional regions in the viral RNA genome as druggable entities
eLife Assessment The study presents a fundamental advance in antiviral RNA research by adapting SHAPE-Map to chart the secondary structure of the porcine epidemic diarrhea virus (PEDV) genome in infected cells and pinpointing structurally conserved, accessible RNA elements as therapeutic targets.
Exploiting functional regions in the viral RNA genome as druggable entities
Abstract RNA-targeting compounds or small interfering RNAs (siRNAs) offer a potent means for controlling viral infections. An essential prerequisite to their design depends on identifying conserved and functional viral RNA structures in cells. Techniques that probe RNA structures in situ have been developed recently including SHAPE-MaP, which has been helpful in analyzing the secondary structures of RNA.
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