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DNA tetrahedron-inspired magnetic hybrid with regular distribution of trypsin for ultra-fast digestion of proteins and glycoproteomics analysis
DNA tetrahedron-inspired magnetic hybrid with regular distribution of trypsin for ultra-fast digestion of proteins and glycoproteomics analysis In large-scale glycoproteomics studies, reducing the experimental duration and enhancing the digestion efficiency are crucial for the experiments.
Preparation of a titanium functionalized polymeric material rich with hydrophilic groups for phosphoproteome and glycoproteome analysis in serum
Preparation of a titanium functionalized polymeric material rich with hydrophilic groups for phosphoproteome and glycoproteome analysis in serum The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene, N, N-methylenebisacrylamide, was polymerized with vinyl phosphate to produce the polymer (denoted as PVME), which contains a variety of hydrophilic groups.
Rapid quantification of disaccharide isomers by derivatization in combination with ion mobility spectrometry in beer and milk
Rapid quantification of disaccharide isomers by derivatization in combination with ion mobility spectrometry in beer and milk The subtle structural variations among carbohydrate isomers pose significant challenges for their identification and quantification. Here, we propose a strategy for rapid identification and quantification of isomeric disaccharides via derivatization with 4-(3-methyl-5-oxo-pyrazolin-1-yl) benzoic acid (CPMP) and analysing by ion mobility spectrometry (IMS).
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