Yinghui Dong
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Efficient prime editors for heritable multiplex precision genome editing in soybean
Abstract Prime editing (PE) is limited by low efficiency in dicot plants. Here we develop an optimized PE system for soybean, GmPEplus, by deleting the RNase H domain, introducing a V223A substitution within the reverse transcriptase domain, inserting a viral nucleocapsid protein between Cas9 and reverse transcriptase, and co-expressing a dominant-negative engineered allele of the endogenous GmMLH1. GmPEplus achieves editing efficiencies of up to 81.3% in stable transgenic lines.
Microstructure, Tribological Properties, and Corrosion Resistance of Multiphase AlCoCrFeNb0.4Ni2.1 High‐Entropy Alloy: The Role of Heat Treatment Time at 800 °C
Conflict of Interest The authors declare no conflict of interest. Data Availability Statement The data that support the findings of this study are available from the corresponding author upon reasonable request. References 1, , , , Lubricants. 2025, 13, 342. 2, , , , , , J. Alloys Compd. 2009, 486, 427. 3, , , , , , , Prog. Mater. Sci. 2014, 61, 1. 4, , , , , , , Tribol. Int. 2021, 163, 107195. 5, , , , , Mater. Des. 2017, 114, 253. 6, , , , , , , , J. Mater. Res. Technol. 2024, 30, 8718.
The Valuation of Mortgages With Partial Prepayment Risk Under the Equal Principal Payment Method
Disclosure The authors have nothing to report. Conflicts of Interest The authors declare no conflicts of interest. Data Availability Statement The datasets supporting the results of this article are retrieved from public data of the Federal Reserve Bank of St. Louis (https://fred.stlouisfed.org/series/DGS10, https://fred.stlouisfed.org/series/MORTGAGE15US). The relevant code is available at https://github.com/Zhou-ht123/VALUATION_code.git.
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