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Modelling collagen fibril self-assembly from extracellular medium in embryonic tendon
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Mmp14 is required for matrisome homeostasis and circadian rhythm in fibroblasts
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Chick tendon fibroblast transcriptome and shape depend on whether the cell has made its own collagen matrix
Our first experiment was to identify genes expressed during tendon development in vivo and then determine which of these genes are expressed by CTFs cultured in collagen-based and fibrin-based TECs. A schematic showing the RNA samples used for microarray analyses is shown in Fig. 1a. Yield and quality of RNA isolated from triplicates of each group were assessed by gel electrophoresis (Supplementary Fig. 1a–c).
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