Yinsheng Li

Abstract Antimicrobial peptides (AMPs) exhibit diverse antibacterial mechanisms. To elucidate the intracellular targets of the novel AMP, EWAMP-R, we generate EWAMP-R-resistant strains of Escherichia coli and Staphylococcus aureus. In E. coli, a nonsynonymous mutation in the clpX gene (1108 C > T) is identified, resulting in an amino acid change in ClpX (R370C). This mutation confers enhanced tolerance to EWAMP-R and induces a “hyperflagellate phenotype” in EWAMP-R-resistant E.

Conflicts of Interest The authors declare no conflicts of interest. Supporting Information Filename Description pol20250414-sup-0001-Supinfo.docxWord 2007 document , 3 MB Data S1. Supporting Information. References 1, , , et al., “Biomimetic High Performance Artificial Muscle Built on Sacrificial Coordination Network and Mechanical Training Process,” Nature Communications 12 (2021): 2916.