Yinsheng Wu

Abstract Protein lipidation is a critical post-translational modification, but the relationship between the fine structure of fatty acids (FAs) and the specificity of lipidation remains largely unexplored. Here, we develop an enzyme-centric chemoproteomic strategy to elucidate the intricate relationships between the key players involved in the protein lipidation process.

1 Introduction Breast cancer is the most commonly diagnosed cancer worldwide with an estimated 2.3 million new cases annual (11.7%), and represents a significant public health concern.[1] The aetiology behind breast cancer involves interactions between environmental, lifestyle, and genetic factors that collectively determine cancer risk.[2] The growth of breast cancer is intimately connected to glucose metabolism, and comparative cohort studies and case-control studies indicate that type 2...

Abstract How cells adapt their gene expression to nutritional changes remains poorly understood. Histone H3T11 is phosphorylated by pyruvate kinase to repress gene transcription. Here, we identify the protein phosphatase 1 (PP1), Glc7 as the enzyme that specifically dephosphorylates H3T11. We also characterize two novel Glc7-containing complexes and reveal their roles in regulating gene expression upon glucose starvation.