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Crystal Structure of Major Envelope Protein VP24 from White Spot Syndrome Virus
The transmembrane truncated VP24 (residues 28-208) was expressed and purified in E. coli BL21 (DE3) successfully. However, a lot of precipitation was appeared while the recombinant VP24 concentration reached above 0.8 mg/ml. Lucky, after screening, with the addition of non-denaturing protein-solubilizing agent NDSB-201, soluble VP24 could reach a concentration of 3.5 mg/ml and yield crystals.
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