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Yongkyun Kim

As seen in: Chemistry World
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Articles

Single-molecule visualization of ROS-induced DNA damage in large DNA molecules

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By Jinyong Lee, Yongkyun Kim, Sangyong Lim
| Chemistry World Verified
We present a single molecule visualization approach for the quantitative analysis of reactive oxygen species (ROS) induced DNA damage, such as base oxidation and single stranded breaks in large DNA molecules. We utilized the Fenton reaction to generate DNA damage with subsequent enzymatic treatment using a mixture of three types of glycosylases to remove oxidized bases, and then fluorescent labeling on damaged lesions via nick translation.
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