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Although a lateral-shear interferometer is robust against optical component vibrations, its interferogram provides information about differential wavefronts rather than the wavefronts themselves, resulting in the loss of specific frequency components. Previous studies have addressed this limitation by measuring four interferograms with different shear amounts to accurately restore the two-dimensional wavefront.
Quantification of the cleavage rate of the mutant loxP sequences by qPCR We assessed the cleavage rate of the mutagenized loxP sequences by qPCR to validate the accuracy of the results of the Illumina sequencing analysis. The results indicated that the addition of various mutations to the RBE can alter the cleavage efficiency of Cre at various rates. However, the sequencing results potentially have a certain bias because it requires PCR during sample preparation.
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