Yumary M. Vasquez

Abstract Advances in genomics have revealed a diversity of nucleocytoplasmic large DNA viruses (phylum Nucleocytoviricota) that infect eukaryotes, but their ecology beyond the oceans remains poorly understood. We recover 208 Nucleocytoviricota genomes, including 205 putative new species and 33 novel virus-host linkages by sampling 87 lakes spanning 30° of latitude from southern Italy to northern Norway.

Abstract Microbial symbiosis drives the functional and phylogenomic diversification of life on Earth yet remains underexplored because of culturing challenges. This study used machine learning (ML) to predict symbiotic lifestyles in more than a hundred thousand microbial genomes from diverse environmental metagenome samples and reference genomes. Predictions were performed using symclatron, an ML framework developed to identify genomic signatures of symbionts.

Abstract Nucleocytoplasmic large DNA and Mirusviricota viruses exhibit taxonomic richness which continually expands due to metagenomic sequencing. Here we curate a database of giant virus metagenome-assembled genomes (GVMAGs V2), comprising 8,508 species-level representatives from 18,727 GVMAGs, a sixfold increase from the previous giant virus phylogenetic frameworks.