Zinger Yang Loureiro

Results To define the cellular landscape of WAT, we profiled five different adipose depots. More specifically, we performed STx and snRNA-seq on biopsies from the abdominal subcutaneous, greater omental, mesenteric (close to the duodenum/jejunum), and mesocolic regions (close to the transverse colon) and from epiploic appendices (on the transverse colon) (Figure 1A). These sites were selected because they are anatomically distinct and accessible during intra-abdominal surgery.

Abstract During aging, adipose tissue within the bone marrow expands while the trabecular red marrow contracts. The impact of these changes on blood cell formation remains unclear. To address this question, we performed single-cell and single-nuclei transcriptomic analysis on adipose-rich yellow bone marrow (BMY) and adipose-poor trabecular red marrow (BMR) from human subjects undergoing lower limb amputations.

Abstract Aging and metabolic diseases are accompanied by systemic inflammation, but the mechanisms that induce this state are not known. We developed a human bone-marrow organoid system to explore mechanisms underlying metabolic-disease associated systemic inflammation. We find that a distinct type of hematopoietic stem cell (HSC) develops in the adipose-rich, yellow bone marrow, which is known to gradually replace the hematopoietic red marrow as we age and during metabolic disease.