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Simultaneous dual-Raman-shift scanning-free stimulated Raman scattering microscopy for label-free biomolecular imaging
Abstract Conventional stimulated Raman scattering (SRS) microscopy requires time-consuming Raman-shift scanning to achieve chemical specificity, limiting its application in real-time biological studies. We present simultaneous dual-Raman-shift scanning-free SRS (SDRSRS) microscopy, which captures different distinct molecular vibrational signatures simultaneously without spectral scanning, achieving more than 10-fold faster imaging while maintaining high chemical specificity.
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