Keywords Animals, Mice, Cell Proliferation, GTPase-Activating Proteins, Intestinal Mucosa, Intestines, Stem Cell Niche, Stem Cells, Wnt Signaling Pathway, stem cells, Wnt, niche, regeneration, Dlg1, Arhgap31, Cdgap, Cdc42, Rac1, organoid, intestine, epithelium, cell death Abstract A central factor in the maintenance of tissue integrity is the response of stem cells to variations in the levels of niche signals.