Abu Jubayer Hossain

Abstract Insulin promotes HepG2 cell proliferation by inducing phosphorylation of the pyruvate dehydrogenase E1α (PDHA1) subunit at Ser293, a mechanism distinct from normal liver tissue. This study investigates how phosphorylated PDHA1 drives hepatocellular carcinoma cell proliferation. We identified eukaryotic elongation factor-1γ (eEF-1γ) as a key binding protein interacting with p-PDHA1 in response to insulin, facilitating their nuclear translocation.

1. Introduction Insulin is a crucial signalling molecule, which has a variety of physiological functions including glucose uptake and glycogen synthesis, as well as the biosynthesis of proteins and lipids in cells. Additionally, insulin promotes cell proliferation and increased insulin, in addition to insulin-like growth factor 1 (IGF1) and IGF2 levels, critically stimulates tumour initiation and progression in insulin-resistant patients [1].