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Akshayaa Venkataraghavan on Muck Rack

Akshayaa Venkataraghavan

As seen in: The Journal of Biological Chemistry
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Streamlining assays of glycosyltransferases activity using in vitro GT-array (i-GT-ray) platform: Application to family GT37 fucosyltransferases

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By Qi Wang, Matrika Bhattarai, Tasleem Javaid, Akshayaa Venkataraghavan
| The Journal of Biological Chemistry Verified
Abstract Numerous putative glycosyltransferases (GTs) have been identified using bioinformatic approaches. However, demonstrating the activity of these GTs remains a challenge. Here, we describe the development of a rapid in vitro GT-array (i-GT-ray) screening platform for activity of GTs. GT-arrays are generated by cell-free in vitro protein synthesis and binding using microplates pre-coated with a N-terminal Halo- or C-terminal GST-tagged GT-encoding plasmid DNA and a capture antibody.
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